Cloning vector(s) without ApaLI site(s)

RVDESHP0 at pwinet.upj.com RVDESHP0 at pwinet.upj.com
Thu Oct 12 08:15:55 EST 1995


Can anyone suggest a cloning vector that does not have an ApaLI restriction 
site (GTGCAC)?  I know that the vectors with colEI replication origin or 
ampicillin resistance marker are unsuitable, because these sequences contain 
ApaLI sites.  I need to clone and manipulate a cDNA at its ApaLI site.  Any 
help would be appreciated.  Thanks in advance.

Rajendra V. Deshpande, Ph.D.
Molecular Biology Research
The Upjohn Company
Kalamazoo, MI 49007 



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