Adding A's with taq

Margon Vandongen vando005 at mc.duke.edu
Thu Oct 12 09:19:50 EST 1995


Jake,

Here is what we do: remove buffer and primers with your method of
choice (we use spincolumns). Add taq buffer and dntp's and 0.5 ul
taq polymerase. At 72 degrees for ten minutes and voila: clone into
TA.(Usually we take 1 ul straight out of PCR reaction).
Yes, somebody asked me once if you need all four dntp's. In our hands
it works better than adding only A's. Good luck,

Margon
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