Cloning vector(s) without ApaLI site(s)
malexeyev at biost1.thi.tmc.edu
Thu Oct 12 13:28:59 EST 1995
In article <07d15010 at pwinet.upj.com>, RVDESHP0 at pwinet.upj.com wrote:
> Can anyone suggest a cloning vector that does not have an ApaLI restriction
> site (GTGCAC)? I know that the vectors with colEI replication origin or
> ampicillin resistance marker are unsuitable, because these sequences contain
> ApaLI sites. I need to clone and manipulate a cDNA at its ApaLI site. Any
> help would be appreciated. Thanks in advance.
You may try vectors of pSU series:
AUTHORS Bartolome,B., Jubete,Y., Martinez,E. and de la Cruz,F.
TITLE Construction and properties of a family of pACYC184-derived cloning
vectors compatible with pBR322 and its derivatives
JOURNAL Gene 102 (1), 75-78 (1991)
More information about the Methods