Cosmid isolation

Steven Goldberg goldberg at
Fri Oct 13 07:58:37 EST 1995

I have used the standard alkaline lysis protocol to isolated cosmids 
with no problems.  The only change I make is to first resuspend the 
bacteria in 0.3 M sucrose/25 mM EDTA/25 mM Tris-HCl pH 8.0 + 2 mg/ml 
lysozyme and incubate for 5-10 min before lysis.  Also, don't vortex the 
tubes, just shake back and forth to mix.


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