Subcloning pcr products

Dr I.M. Clark imc1001 at cus.cam.ac.uk
Fri Oct 13 06:50:49 EST 1995


We have produced an approx 500bp pcr product with a SalI site at 
one end and a BamHI site at the other.  We have tried to cut and
subclone this into a Sal/Bam cut vector with no success.
Is there a particular problem with cutting such pcr products
or using these particular enzymes??  Any hints or advice would 
be appreciated.
Thanks
Ian

--
Ian M. Clark
Rheumatology Research Unit,
Addenbrooke's Hospital,
Cambridge. UK.



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