rrohan at world.std.com
Fri Oct 13 23:22:14 EST 1995
Milner and Sutcliffe (NAR 11:5497 1983) wrote "One class I clone, p1B15,"
(latter found to be cyclophilin) " has subsequently been used as a
hybridization probe with many other tissues (adrenal, spleen, testes, heart,
muscle, thymus, pituitary and intestine and various rodent cells inculture),
and its corresponding mRNA is found in approximately the same concentration
ineach of these cell types (data not shown)." On the other hand, Jakubowski
et. al. (Endocrinology 128:2702-2708 1991) noted slight (less than two-fold)
changes in cyclophilin abundance in rat brain between 1 and 90 d.o.. Also,
cyclophilin expression in pig granulosa cells has been found to be regulated
by phorbol esters (Lahav et al. Biology of Reproduction 52:972 , 1995).
The bottom line, everything probably changes at some time or another. Use an
external standard if you want to be absolutely positive.
rey garcia <rey.garcia at stonebow.otago.ac.nz> wrote:
>Further to my posting 2 days ago, I found an article by Freemark et al
>(1995 - Journal of Endocrinology) where they used cyclophilin as an
>internal standard in their RT-PCR to compare expression of the prolactin
>receptor gene in fetal tissues. Does anyone know if cyclophilin
>expression is relatively invariant in both fetal and adult tissues? I'm
>only after comparative expression (as opposed to absolute quantitation)
>and I want to know if cyclophilin is a good standard to compare
>expression of "my" gene in tissues such as fetal ovary, non-pregnant
>ovary, fetal testis, adult testis, fetal liver, adult liver, adult
>spleen, adult adrenal, adult cerebral cortex, adult kidney and bone
>marrow. Also, are there tissues where cyclophilin is not expressed? Any
>useful info on cyclophilin expression will be greatly appreciated.
>Please e-mail replies to:
>rey.garcia at stonebow.otago.ac.nz
More information about the Methods