PCR and Gels

Andy Kravetz akravetz at Walden.MO.NET
Fri Oct 13 18:07:05 EST 1995

Hello, I am doing a PCR of a fragment that is 375bp long but I am 
consistently getting a band at 250bp. I have ranged the annealing from 45 
up to 69 where all the false bands disappear and only the 250bp is left. 
My question is this: Can a linear dsDNA PCR product run smaller than it 
really is? If so, why. If not, what could I do to perhaps improve on my 
system. I am using 300ng template and about 100pmol primer. Thanks


Andy Kravetz
Department of Biochemistry and Molecular Biphysics
Washington University
St. Louis, Missouri
akravetz at walden.mo.net

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