ABI's Taq FS

Richard R. Hardy hardy at mighty.fccc.edu
Sat Oct 14 13:19:13 EST 1995


In article <45k6c3$ie1 at nntp3.u.washington.edu>, jason2 at u.washington.edu
(Jason Seto) wrote:

>Has anyone used the Taq FS from ABI and found they have not had to purify
>the sequenced product prior to electrophoresis on a 3373/377?
>
>Does the EtOH ppt step work, or do you lose the first few bases?
>or do people still use the spin columns to purify off the excess dyes,
>regardless of ABI's claims that you don't need this step with their new 
>enzyme/kits?

We have been getting good results with the new Taq FS kit from ABI; we
compared sequences from the old kit (w/spin column) to that with new kit
(EtOH ppt) and got equivalent or (in most cases) better sequences on an
ABI 377.
____________________________________________________________________________
Richard R. Hardy                       Member, Institute for Cancer Research           
Fox Chase Cancer Center                Tel:    (215) 728-2463
7701 Burholme Ave.                     FAX:    (215) 728-2412
Philadelphia, PA 19111                 E-MAIL: R_HARDY at fccc.edu



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