Size-Fractionation of DNA

colvin at ouvaxa.cats.ohiou.edu colvin at ouvaxa.cats.ohiou.edu
Mon Oct 16 10:43:50 EST 1995


Before subclonning ds DNA generated by PCR into a plasmid, I would like to 
remove small DNA fragments.  The template used for PCR was a subtracted cDNA
library, so there are many different sized fragments present.  One method
that I am aware of is to use a sepharose CL-4B column.  Have others used this
method with success?  Are there other methods that are simple, but still give
good recovery?  Thank you.
Bob Colvin, Dept. Biological Sciences, Ohio Univ. Athens, OH, USA
colvin at ouvaxa.cats.ohiou.edu



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