non-heat sterilization - suggestions?
logan at msdos.ensam.inra.fr
Fri Oct 13 03:48:49 EST 1995
*Hello. We use big plastic trays (25cm^2) for plating E. coli and lambda
*phage. They are rather expensive (circa $5 ea.), and we use a lot of them.
*I need a method of sterilizing them, after they have been cleaned, for
*reuse. They cannot be reused. Does anyone have any brilliant (heck - any)
*suggestions! ... :)
*Greg Stuart gstuart2 at sol.uvic.ca
*Centre for Environmental Health, 9865 W. Saanich Rd.,
*Sidney, B.C., CANADA. V8L 3S1. FAX: (604) 656-8148 TEL: (604) 656-8146
Yes it is possible.
What I do is, after discarding the agar/bacteria or phage already there into a
bag to be killed, I soak the dishes in bleach solution for a while then give
them a good scrub out, I use very hot water (this washing is probably common
sense, but I'm detailing it in case there is some magic!). Then once they're
dried I put them into a sterile flow hood with the inside of both half upwards,
close the hood, then switch the UV light on for 30 min. (maybe shorter times
will work, I haven't tried). Turn on the sterile flow, before switching off the
UV then carefully put the lid on the box. What I did the first time to see if
this had worked was pour LB media into one of them and incubate O/weekend.
nothing grew, so I figured it was OK. I have been doing this fairly successfully
since, and have reused the same boxes at least 3x.
Anyway, I hope this works for you
Logan at msdos.ensam.inra.fr
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