SSCP PAGE problems
a.ola at umds.ac.uk
Mon Oct 16 11:15:24 EST 1995
I'm trying to screen some DNA samples for mutations using the SSCP
method. After running my 5% PAGE + 10% glycerol gel for 15hrs at room
temp my gels have refused to stick onto the Whatman paper and every
attempt to dry the gel ends up losing the gel. My gel is made up as ffws
16ml 50% glycerol
8ml 50% Acrylamide Bis(49:1)
4ml 10X TBE
0.48ml 10% APS
Running buffer used is 0.5% TBE.
I would be very grateful if anyone could help me out as I'm running
Please mail replies, thank you.
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