fluore-dATP labelling mix for ALF auto sequencing

"Marianne Leverone ", BIO leverone at CHUMA.CAS.USF.EDU
Mon Oct 16 07:49:18 EST 1995


Hi methods and reagents:  Has anyone used Pharmacia's fluore-dATP labelling 
mix with a non-fluorescent primer to sequence with the ALF DNA 
sequencer?  How did it work compared to using the ALF 
pre-fluorescein-labeled primers?  I'm trying to determine whether using a 
pre-labelled primer gives better results than labelling with the 
fluore-dATP labelling mix.  Custom made fluoescein-labeled primers are 
expensive but if they yield better results, they may be worth the price.

				Marianne Leverone
				Univ. of South FLorida
				Tampa, FL  



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