Size-Fractionation of DNA
cwen at aux.btny.purdue.edu
Mon Oct 16 13:49:16 EST 1995
colvin at ouvaxa.cats.ohiou.edu wrote:
>Before subclonning ds DNA generated by PCR into a plasmid, I would like to
>remove small DNA fragments. The template used for PCR was a subtracted cDNA
>library, so there are many different sized fragments present. One method
>that I am aware of is to use a sepharose CL-4B column. Have others used this
>method with success? Are there other methods that are simple, but still give
>good recovery? Thank you.
>Bob Colvin, Dept. Biological Sciences, Ohio Univ. Athens, OH, USA
>colvin at ouvaxa.cats.ohiou.edu
I am using Sephacryl 400HR which gives me a very good resolution and separation.
More information about the Methods