actin as internal standard in RT-PCR

Junhyong junhyong_kim at quickmail.cis.yale.edu
Tue Oct 17 14:31:15 EST 1995


There's usually so much rRNA that it comes through most poly-A 
purifications. I've used it as a standard and it works fine with poly-A 
purified mRNA. One advantage is that these sequences are so conserved 
that you can make one set of primers and use it for practically 
anything.

Junhyong Kim





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