quantification of apoptosis

P. Friedl di58 at pop.th-darmstadt.de
Tue Oct 17 05:31:36 EST 1995


In article <45jm39$aul at ujf.ujf-grenoble.fr>, Abdelhamid Benazzouz <Abdelhamid.Benazzouz at ujf-grenoble.fr> says:
>
>I'm inducing apoptosis on PC12 but a part of effect is probably necrosis.
>I need a relatively simple method to quantify the number of apoptotic cells
>(without using flow cytometry)
>
>Thank you for answer
>
>
>David BLUM


Hello,
DAPI-staining of cells and counting the fraction of cells with microscopically
visible nuclear fragmentation yields the same results as flow cytometry.
P.F.



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