Help on nested deletion system
Tue Oct 17 14:03:41 EST 1995

In article <460lpo$p9h at>, sa95078 at
(sa95078) wrote:

> Hi, can anyone give me some suggestion for a reliable unidirectional
> nested deletion system.
> Thanks in advanced.
> James

Hi there,

I have had success using a DNAse deletion strategy which can be found as

Lin, Lei and Wilcox (1985) 
An improved DNA sequencing strategy
Analytical Biochemistry 147: 114-119

The only modification I made to the procedure was to replace all the DNA
precipitation steps with Promega's magic (now wizard) cleanups.  This
increased my yield of colonies about 1000-fold.  I'm sure other DNA clean
up columns would also work (no affiliation with Promega etc).

Hope this helps

Kim Snowden
ksnowden at

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