Help on nested deletion system
Tue Oct 17 14:03:41 EST 1995
In article <460lpo$p9h at oac4.hsc.uth.tmc.edu>, sa95078 at odin.mda.uth.tmc.edu
> Hi, can anyone give me some suggestion for a reliable unidirectional
> nested deletion system.
> Thanks in advanced.
I have had success using a DNAse deletion strategy which can be found as
Lin, Lei and Wilcox (1985)
An improved DNA sequencing strategy
Analytical Biochemistry 147: 114-119
The only modification I made to the procedure was to replace all the DNA
precipitation steps with Promega's magic (now wizard) cleanups. This
increased my yield of colonies about 1000-fold. I'm sure other DNA clean
up columns would also work (no affiliation with Promega etc).
Hope this helps
ksnowden at bio.tamu.edu
More information about the Methods