RNA and Protein from the same sample

straubek at VAX.CS.HSCSYR.EDU straubek at VAX.CS.HSCSYR.EDU
Tue Oct 17 12:58:12 EST 1995


>Does anyone out there know of a method that allows you to prepare RNA and
>Protein from the same sample.  We are hoping to analyse the RNA by
>Northerns and the protein by Westerns.
>
>Thanks for your help
>
>Sue Green

Sue,

Our lab uses TRIzol from Gibco. We have, on several occasions, used it to
obtain RNA, protein, and genomic DNA from the same sample. It is very
simple to use and we have always obtained high quality results.

Karen Straube-West, Ph.D.
Department of Pharmacology
SUNY Health Science Center
750 East Adams Street
Syracuse NY  13210
phone:(315)464-7959
fax:  (315)464-8014
e-mail: straubek at vax.cs.hscsyr.edu





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