"Zap" entire cDNA library?
jgraham at bronze.ucs.indiana.edu
Tue Oct 17 11:22:46 EST 1995
Can anyone comment on the ability of excising an entire Bluescript
library from a Lambda ZapII cDNA library?
Preliminary experiments suggest that as many as 5 of 12 inserts
inhibit either the "Zap" M13 packaging or are not as well propagated
in plasmids a in the Lambda vector.
Stratagene seems unable to grasp the distinction in the burden
of maintaining a plamsid vs a recombinant Lambda. The Short
reference describing Lambda Zap shows 12/12 excisions, yet the
orginal Lamda sources were not mapped. Hard to beleive in light of
my preliminary observations.
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