bbur at wpo.nerc.ac.uk
Tue Oct 17 14:48:03 EST 1995
In article <460733$d3s at whitbeck.ncl.ac.uk> n4522701 <R.Guy.Reeves at ncl.ac.uk> writes:
>From: n4522701 <R.Guy.Reeves at ncl.ac.uk>
>Subject: Cycle sequencing
>Date: 17 Oct 1995 12:20:51 GMT
>I am thinking of trying out Cycle sequencing of PCR products.
>Does any body have advice or tips on what is an effective kit,
>is cycle sequencing tricky to do??? How little DNA do I need
> to be successfl.
>Dpt of Biological & Nutritional Sciences
>Newcastle upon Tyne
>Fax (0) 191 222 8684
>R.Guy.Reeves at ncl.ac.uk
I used an automated system and Applied Biosystems PRISM DyeDeoxy Cycle
It's a doddle, easier to set up than a PCR BUT if I was going to do it again I
would clone first. Heck, I AM going to do it again :-).
You need very little template, 100-200 ng springs to mind.
Don't know about manual systems, I imagine the only complication is doing it
in four tubes in stead of one? Correct me someone if I'm wrong.
Good luck! And give my love to the Bacchus, the Red House, the Duke of York...
*sigh* days gone by....
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