nian at nian at
Tue Oct 17 22:12:23 EST 1995

Hi, netter,

Is anybody using acid-phenol to remove the nick or linearized DNA from circular 
dsDNA.  What is the critical step?  How lower the pH can be? What is the 
mechanism ?  I tried many times but no success at all. Any suggestions will be 

Nian G.

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