Acid-phenol

nian at pc-10.camden.usyd.edu.au nian at pc-10.camden.usyd.edu.au
Tue Oct 17 22:12:23 EST 1995


Hi, netter,


Is anybody using acid-phenol to remove the nick or linearized DNA from circular 
dsDNA.  What is the critical step?  How lower the pH can be? What is the 
mechanism ?  I tried many times but no success at all. Any suggestions will be 
appreaciated.  


Nian G.




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