troianovsk_s at MSDISK.WUSTL.EDU
Tue Oct 17 17:52:23 EST 1995
> To: methods-and-reagents at net.bio.net
> From: wjl at hopper.unh.edu (Woo-Jai Lee)
> Subject: glucose
> Can glucose be autoclaved in a medium like 2X YT? or should it be
> sterilized separately?
> Any comments would be appreciated.
> Woo-Jai Lee
But I also never sterilized (filtered through 0.22 filter) it separetly
too. I just simply add it to autoclaved medium until desered concentration
(2%) is reached. In any way there should be antibiotics in the medium, and
I hardly expect a lot of bugs, if any, in dry powder.
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