AGAROSE MICROBEADS

[d]

TO ANYONE THAT CAN HELP
I AM CURRENTLY TRYING TO CONSTRUCT A RICE GENOMIC YAC LIBRARY AND THE 
HIGH MOLECULAR WEIGHT DNA THAT I HAVE AVAILABLE IS STILL WITHIN NUCLEI
AND SUSPENDED IN AGAROSE MICROBEADS.I SEEM TO BE HAVING GREAT PROBLEMS 
IN GETTING THE DNA OUT OF THE BEADS SO THAT I CAN DO PARTIALS ON IT,ETC
ANY ADVICE OR PROTOCOLS FOR EXTRACTION FROM THESE BEADS WOULD BE VERY 
USEFUL

THANKS IN ADVANCE

SIMON DAY
UNIV OF CAMBRIDGE
DEPT OF GENETICS
CAMBRIDGE