[Q] PCR gives extra band - heteroduplex formation?
vblasch at gwdg.de
Wed Oct 18 03:18:38 EST 1995
We do quant. PCR just the same way and I do see heterodimers just between
the larger (1149 bp) and the smaller (962 bp) band, so I presume the
heterodimer runs according to its actual size, which should be around
(1149+962)/2=1055 bp, just what I am getting.
I have no idea where the band larger than the large product in the
original posting might come from.
Dr. Volker Blaschke
Depts. of Dermatology, Biochemistry
Georg-August-University, Goettingen, Germany
Email: vblasch at gwdg.de
Tel: xx49 551 39 5959 / 5978
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