Blunt end ligation problems

Dr. P.A. de Zoysa pdezoysa at hgmp.mrc.ac.uk
Thu Oct 19 05:18:30 EST 1995


Try 37 deg C for 4 hrs with 1 u of BRL ligase and buffer.  (The buffer has PEG in it
and if you need more details you can get it from BRL).  The other modification you should carry out is to blunt-end before gel purification (ie immediately after RE
digestion See Pages F2-F5 in Sambrook et al., (1989).

Hope thi helps,

Priyal de Zoysa.








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