diabetes at sunmail.lrz-muenchen.de
Thu Oct 19 14:23:28 EST 1995
Hi. Woo-Jai Lee
We are adding previously sterilized Glucose to our media (DMEM, M-199, RPMI) just when we are
preparing the solutions for work.
Glucose is dissolved in water and then sterilized for 20 minutes at 120°C in a steam autoclav.
To prevent excess formation of brown products, we open the autoclav still hot (be VERY CAREFUL
!!!), close the bottles and put them into a cold room, where the solutions are stored until
use, but not more than a few weeks. That's a "traditional". I have no reference in the
literature for that, but if anyone knows, please tell me!
Hope this will help.
Have a nice day!
wjl at HOPPER.UNH.EDU (Woo-Jai Lee) wrote:
>Can glucose be autoclaved in a medium like 2X YT? or should it be
>Any comments would be appreciated.
Institute for Diabetes Reserch
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Phone +49 (89) 30 79 31 24
Fax +49 (89) 30 81 733
email u7k0201 at sunmail.lrz-muenchen.de
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