ethidium bromide alternative?
workshop at dnatech.com
workshop at dnatech.com
Thu Oct 19 13:04:39 EST 1995
> horton at biosci.cbs.umn.edu (Robert Horton) writes:
> Hector Cruz-Lopez (pezmen at gate.net) wrote:
> : Hoeschst staining is pretty similar to EtBr, also fluorescent but less
> : hazardous (with adversions in mind!). E-mail if interested and I'll get you
> : the recipes.
> How can you say it's less hazardous? As far as I have been able to tell, the
> worries about EtBr hazards are largely theoretical. I understand it has been
> shown to be mutagenic in bacteria (though I don't have a reference for that),
> but it has not been shown to be mutagenic, much less carcinogenic, in mammals.
> If I am wrong about this, please correct me. I have not been able to find
> good info on this subject in Medline, but maybe you all know something I
> I would think that ANY intercalating dye should be treated with respect
> for its mutagenic potential, on theoretical grounds. Or any DNA-binding dye,
> for that matter. But why do you worry about EtBr more than about other dyes?
> In fact, Ethidium Bromide has been used for years in veternary medicine
> to kill trypanosomes. I don't know how much it takes to kill a trypanosome,
> but I bet its a bunch. I realize that just because people have used something
> for a long time doesn't mean that they actually know a lot about it, but
> still, EtBr has been used in large quantities in mammals for a long time,
> and, as far as I can tell, has never been shown to be carcinogenic. Can the
> same be said for alternative dyes? And scientists have used it for years, too.
> Has anybody out there ever had an adverse reaction to the stuff? Personally,
> I think it would be wiser to stick with the devil we know...
> I don't mean to preach, but this topic really bothers me because it is
> a superstition among scientists. If someone can point to an actual study
> that shows alternative dyes are safer than EtBr, please let me know.
> Robert M. Horton (PhD!) /\ "Crash programs fail because of the theory that
> U of M Dermatology Dept || with nine women pregnant you get a baby a month"
> Box 98 UMHC, 4-154 PWB /||\ -W. von Braun. Disclaimer: "Bob who?"
> Minneapolis, MN 55455 ^^ horton at biosci.cbs.umn.edu (612)625-8941
I have taken quite a liking to SYBR green as an alternative to staining with ethdium bromide. We get ours from FMC Corporation
in Maine (you know, the agarose people.....)
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