pcr-primers

Volker Knoop knoop_v at mpimg-berlin-dahlem.mpg.de
Fri Oct 20 18:00:37 EST 1995


In article <Jan.Svensson.24.307FA343 at mikrob.slu.se>,
Jan.Svensson at mikrob.slu.se (Jan Svensson) wrote:

>hi!
>
>I bought pcr primers to amplify cDNA and clone the fragments into an 
>expression vector. When i sequence my clones i have seen a lot of errors
>when i read over the pcr-primers. 1 primer has in one case 2 substitutions and 
>in another 3 substitutions yet another primer has 2 substitutions. 
>All the sequence observation obtained sofar shows that the sequence of the 
>primers not are correct.     ...     The company claims that this is very
common ?
>and seems to be recluctant to the idea that they have made bad primers.
>>Has anyone here had any similar problems or do u have any ideas please post 
>answers to this group.

I do not think this is or should be common.
I have only seen sequence variation in primers where I wanted to see them,
i.e. when using degnerated oligos.
Changing the company would be my method of choice.

-- 
Volker Knoop
IGF Berlin Dahlem
14195 Berlin
phone +49-30-830007-48 Fax -36
e-mail: KNOOP_V at MPIMG-BERLIN-DAHLEM.MPG.DE



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