****32-P PCR generated probe***

Rafael Maldonado rafael at howard.genetics.utah.edu
Fri Oct 20 15:58:15 EST 1995


On 19 Oct 1995, b.schonhoft wrote:

> I'm looking for a protocol to make a 32-P labelled probe.  I'm want to use
> PCR to generate the probe:
> 
> size: 1kb 
> 32-P ATP @ 3000uCi/mmol   (tried various amts.)

Have you been using ATP or dATP? It is alfa or gamma labelled?

Just curious...

> 200um dG/C/TP final
> HOW much cold ATP?????


	Rafa


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