Transient transfection

Mirta Grifman mirtag at leonardo.ls.huji.ac.il
Sat Oct 21 18:20:14 EST 1995


CYCS at MUSICA.McGill.CA (Caroline) wrote:
>Hi!
>
>   I'm having a lot of trouble with my transient transfection of NIH-3T3
>cells using calcium phosphate precipitation.  I'm trying to do luciferase
>assay to study a promoter.  I been trying transfection using the positive
>control (Pomoter vector from promega) and a beta-galactosidase gene
>(lacZ-III) and got neither luciferase or galactosidase activity detected.
>I heard that NIH-3T3 cells are a pain in the ... to transiently
>transfect.  If any of you can give me a magic protocol or any suggestion
>for transient transfection, let me know.
>
>Thank you very much
>Caroline
>E-mail: CYCS at MUSICA.McGILL.CA

I use Lipofectamine (GIBCOBRL) to transfect PC12 cells. It is a very efficient method. If you want to try it I can give you a detailed protocol. Good luck!
Mirta.





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