pcr-primers

j. boyson at primate.wisc.edu
Sat Oct 21 10:31:59 EST 1995


But-- just for argument's sake, what if the sequence the PCR primers 
were designed from was incorrect? or variable? Then, the primers could 
have a few mismatches. Thus, when the mismatched primer synthesized its 
strand, that strand will contain "incorrect" sequence, but when the 
other primer synthesizes its strand, that strand will contain "correct" 
sequence data, because it's copying the 'real' sequence. 

Could this happen?





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