E. Coli Transformation
goldberg at bms.com
Mon Oct 23 10:21:24 EST 1995
You might want to check your pipetters--there may be some stuff growing
in the barrel that gets ejected when you use them in your
transformations. If you have them, try using aerosol-resistant pipette
tips when you perform your experiments. I have also seen instances
where the ligation mix is contaminated but only grows when inoculated
into rich medium.
Hope one of these ideas help.
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