E. Coli Transformation

Steven Goldberg goldberg at bms.com
Mon Oct 23 10:21:24 EST 1995


You might want to check your pipetters--there may be some stuff growing 
in the barrel that gets ejected when you use them in your 
transformations.  If you have them, try using aerosol-resistant pipette 
tips when you perform your experiments.  I have also seen instances 
where the ligation mix is contaminated but only grows when inoculated 
into rich medium.

Hope one of these ideas help.

Steve Goldberg





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