ECL Optimisation

John K. Troyer jtroyer at
Mon Oct 23 08:53:46 EST 1995

On 22 Oct 1995, Seth Corey wrote:

> do people put in azide in their primary ab? and will it not
> interfere with ECL?
> thanks,
> seth


I routinely use sodium azide in my primary and my blocking buffer, which I 
also use neat to dilute the secondary antibody and have no trouble with 
interference of ECL.   While azide may be able to inhibit HRP to some extent, 
by the time the blot is washed, there is little to no azide left on the blot 
and has no effect on the peroxidase reaction.



John K. Troyer, PhD
University of Maryland School of Medicine
Department of Biological Chemistry
jtroyer at

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