cross-species hybridization

Chris McIntosh bb203 at FreeNet.Carleton.CA
Sun Oct 22 20:03:15 EST 1995


Hi folks.  I'm having a problem with my cDNA library screening, and I
believe it has something to do with my probe hybridization.  My probe may
be as low as 50% identical.  I'm using low stringency conditions (55
degrees without formamide, 5 X ssc wash).  But I still cannot pick up a
signal from the plaque lifts or from a Southern of genomic DNA.

Any suggestions?

CMc

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