bb203 at FreeNet.Carleton.CA
Sun Oct 22 20:03:15 EST 1995
Hi folks. I'm having a problem with my cDNA library screening, and I
believe it has something to do with my probe hybridization. My probe may
be as low as 50% identical. I'm using low stringency conditions (55
degrees without formamide, 5 X ssc wash). But I still cannot pick up a
signal from the plaque lifts or from a Southern of genomic DNA.
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