Problems with Promega pGEM T-vector
mbzspd at granby.nott.ac.uk
Tue Oct 24 12:30:01 EST 1995
We have used the Promega kit in the past and it worked fine. I take
it your are using their ligase buffer/ligase ?? Have you checked the
control insert is actually present? If you did everything the way the kit
suggests, I would talk to Promega and get a new kit/your money back. That
would be easiest and save you time. We use our own 'T-vector' in the lab
now. It's awesome, extremely cheap to make and virtually
'idiot-proof'....thats why I can do it!
If you want a reference for the home-made method we use, drop me a line
and I'll email you one (it's a Word document but I can send it as plain
text if thats better).
Dr. Simon Dawson TEL:+44 (0)115 9249924 ex. 44789
Dept. of Biochemistry FAX:+44 (0)115 9422225
Queens Medical Centre Email:Simon.Dawson at .nott.ac.uk
Clifton Boulevard URL: http://www.nott.ac.uk/~mbxspd/Simon.html
U.K. "Back off man, I'm a scientist!" - Bill Murray.
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