GST fusions - please, help

Duncan Clark Duncan at genesys.demon.co.uk
Tue Oct 24 12:01:27 EST 1995


In article: <46ivgu$7m at grandcanyon.binc.net>  Jennifer Lyon <JLyon at novagen.com> 
writes:
> 
> 
> Actually, BL21 is both lon- and ompT-. It is however, recA wildtype.
> It is good for protein expression, though I would not recommend growing 
> plasmid in it. 

I stand corrected. I hadn't realised that E.coli B strains were 
automatically lon-. I wonder why they don't form mucoid colonies like all 
K12 genuine lon- strains do?  

Duncan
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