GST fusions - please, help
Duncan Clark
Duncan at genesys.demon.co.uk
Tue Oct 24 12:01:27 EST 1995
In article: <46ivgu$7m at grandcanyon.binc.net> Jennifer Lyon <JLyon at novagen.com>
writes:
>
>
> Actually, BL21 is both lon- and ompT-. It is however, recA wildtype.
> It is good for protein expression, though I would not recommend growing
> plasmid in it.
I stand corrected. I hadn't realised that E.coli B strains were
automatically lon-. I wonder why they don't form mucoid colonies like all
K12 genuine lon- strains do?
Duncan
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