ypl at uhura.neoucom.edu
Tue Oct 24 13:04:19 EST 1995
Does anybody have the experience on PKC (protein kinase C) assay by
using traditional assay method or PKC assay kit (from Gibco)?
Which one is more sensitive?
Does anybody use tumor promoter-TPA (also known as PMA) to stimulate
PKC activity? I tried to use 1 microM for 10 min incubation in brain
slices, but somehow the result is different from other papers (suppose
it should stimulate large increase of membrane-bound PKC and decrease
cytosolic PKC). Please give me any suggestion or advice. Thank you
e-mail: ypl at uhura.neoucom.edu
More information about the Methods