Fold Induction with lacUV5 promoter

taylor patel_p at bms.com
Wed Oct 25 10:04:39 EST 1995


Hi:

	What fold induction is expected with a fully induced lacUV5 promoter.
I understand, that induction may vary with certain growth conditions and the
strain of E. coli used.  However, I was wondering the approximate number
(2X, 4X, 8X or 50X) between the level of expression without an IPTG being
present vs. >200 mM IPTG present in the medium.

Thanks in advance

Prem

e-mail:     Patel_P at BMS.com



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