PCR with 35S/32P-dATP

Markus Piotrowski Markus.Piotrowski at rz.ruhr-uni-bochum.de
Tue Oct 24 17:51:18 EST 1995


tedm at darkwing.uoregon.edu (Ted M.) wrote:
>In article <18OCT95.06573938 at tifrvax.tifr.res.in>,
>nevil at tifrvax.tifr.res.in (Nevil J Singh) wrote:
>
>> Hi,
>> Is it possible to use 35S or 32P dATP instead of cold dATP in PCRs...

>> Thanx,
>> -nevil
>
>Nevil,
>.... As for the 35S, you may note that at least one PCR machine >manufacturer advises against using 35S in their machine, they say the >volitile component (?) seems to go through the tubes! The result is a >chronic 35S contamination problem-yuch!
>good luck.
>
>regards,
>Ted Michelini
>Institute of Molecular Biology
>University of Oregon
>tedm at darkwing.uoregon.edu


Hi Nevil, hi Ted,

This one manufacturer is right. In our lab we have no problems with
contaminations of our PCR machine when we used 32PdATP for labelling of
PCR-probes but get usually a contamination when making differential
display with 35SdATP.

Best regards

Markus




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