Isopropanol precipitation of DNA.

Dr.Sailesh Surapureddi SaiSu at MCB.LiU.SE
Wed Oct 25 15:37:40 EST 1995


Hi,
	Dont use any salt, just mix 0.75vols of isopropanol and 
immediately centrifuge and you get a unvisable DNA pellet 
(centri-hard), this is good for sequencing. 
	The other suggestion let all the solvents (isopropanol and your 
DNA sample) be at room temp. this helps you in preventing the 
precipitation of salts.



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