Subcloning of PCR products.

yeast at imtech.ernet.in yeast at imtech.ernet.in
Wed Oct 25 21:05:19 EST 1995


Hi Ian, 
      Different restriction enzymes would require different no.
of nd. preceding their restriction site for effecient cleavage.
Ckeck out if you have sufficient no. of nd. for your REs in the 
primers.

You could also try using phosphorylated primers, followed
by concatamerization of your PCR product.  This would 'internalise'
the terminal RE sites, enabling the REs to cut more effeciently.

Good luck with that !
S R   



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