suitable protease negative, lacIq E.coli

sl3wx at cc.usu.edu sl3wx at cc.usu.edu
Thu Oct 26 15:34:36 EST 1995


Hi Nettors,
I would appreciate a recommendation for a protease negative strain of E.coli
that is lac Iq and a suitable source supplying the same.
I have my gene cloned into plasmid vector pIN-III-ompa 2 that has the 
lpp5-lac promoter and suspect the reason I am not getting protein expression
is proteolytic degradationof the expressed protein.
Would welcome any thoughts on the subject.
Thanks.

Ventris.




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