Sequencing PCR poduct w/ PRISM

Dale Levitzke Dlevitzke at medicine.newcastle.edu.au
Fri Oct 27 01:10:12 EST 1995


I've been operating an ABI 377 at the Biomolecular Research Facility in 
Newcastle and have had good results sequencing PCR templates.  As a 
general rule the template must be clean (Wizard PCR kits or Geneclean 
work well) and use 100ng of PCR template per 300bp of product that you 
wish to sequence, go for it.....





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