Perfect-match PCR enhancer

Carolyn Calnan c-calnan at petermac.unimelb.edu.au
Thu Oct 26 01:41:02 EST 1995


Does anyone know a home made substitute for "Perfect Match" enhancer?
I am having problems amplifying a 1.4 kb fragment of the mouse p53 gene. 
It works on genomic DNA extracted from some cell lines but not for 
others. The DNA looks fine on agarose gels. It amplifies with iunrelated 
primers and with p53 primers plus PME, but PME is so expensive !!!
Any sugestions?




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