multiple sequences from Edman degrad

[Shaun D. Black]

Hi All,

     C478SCB at SEMOVM.SEMO.EDU (Bilbrey Robert) asked about how to get sequence
information from many peptides sequenced manually.  Well, I believe it is
quite do-able.  I've personally sequenced about a dozen peptides at a time
manually down to about the 20 pmol level and have run about 15 cycles
successfully.  I used Dr. George Tarr's "Manual Edman Sequencing System".
Check out his nicely written chapter (p. 155) in "Methods of Protein
Microcharacterization: A Practical Handbook (J.E. Shively, ed.) [Humana
Press, Clifton, N.J. USA, 1986, ISBN #0-89603-090-3].  He also details
CNBr (and other) cleavage methods that could be applied to gain internal
sequence info.  I hope this helps- it's the best reference I know of.
Best regards,  Shaun

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  = Shaun D. Black, PhD   | Internet address:     shaun at jason.uthct.edu = 
  = Dept. of Biochemistry | University of Texas Health Center, at Tyler = 
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