beta-gal staining of BHK21 cells

M. Alexeyev malexeyev at biost1.thi.tmc.edu
Fri Oct 27 11:01:50 EST 1995


Dear netters:  
I am staining BHK21 cells transfected with plasmid encoding
beta-galactozidase. As a result I can see blue cells in both negative
control and in my experiment (the numbers are almost equal). My solutions
are made in PBS (pH7.3). Any remedies/ideas-suggestions will be
appreciated.
Thanks in advance.

M. Alexeyev



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