is quantitative blot possible ?

Stefan Bäckström stefanba at bio.embnet.se
Fri Oct 27 04:49:54 EST 1995


Hi there.

Does anyone know of a good way of transferring proteins using western in a
quantitative way ? 

It seems difficult to transfer a broad MW range. If you run it for a short
period, small molecules will migrate onto the ns-filter. But if you want
to blot bigger proteins also, (longer time) the small ones will migrate
through the nc filter and onto the filter paper beneath. 

Any advice ?

Stefan Backstrom,
Inst of developental neurobiology, Uppsala



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