TRANSFECTION of Human Monocytes

Dirk Jochmans dirk.jochmans at chaos.kulnet.kuleuven.ac.be
Fri Oct 27 04:32:36 EST 1995


I would like to investigate a promotorsequence which is followed by
a beta-galactosidase reportergene in a pUC-like plasmid.
I hope to transfect this plasmid in human peripherial monocytes and not in stable cell lines
because I already noticed that the gene of interest can not be induced in THP-1 or MonoMac cells.
If there is anybody how knows good transfection conditions for transient transfection
of human monocytes please send them to:

dirk.jochmans at chaos.kulnet.kuleuven.ac.be

thank you



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