Isolating areas flanking a gene
Brad_Nicholson at hlthsci.med.utah.edu
Mon Oct 30 17:34:30 EST 1995
In article <4738s1$qiq at er6.rutgers.edu>, driscoll at eden.rutgers.edu (William
> I would like to isolate the areas flanking a particular cloned gene from
>strand isolation idea sniped<
>Also any other alternate ideas for isolating the areas 5' of a gene would
be >interesting to hear.
>Thanks in advance,
>driscoll at eden.rutgers.edu
Have you considered inverse PCR (IPCR)? I have tried it to isolate DNA
flanking a Tn10 insertion in bacteria. WHile plant genomes are much more
complex, it may work. You can do a literature search to see if anyone has
used IPCR in plants or you can look at some stuff which has worked in
bacteria. There is a reference for inverse PCR off of Tn10 in Infect.
Immun. (1994) 62:1623-1630. This is what I used as a starting point and it
worked, first time. :) Email me if you have questions, and I'll try to
help you as much as I can.
Brad Nicholson |"If it worked the first time,
Department of Pathology | it wouldn't be research."
University of Utah | ...Brad Nicholson
Salt Lake City, UT 84132 | My opinions are solely my own.
Brad_Nicholson at hlthsci.med.utah.edu |
or: (801)-581-4365 | iligitimi non corborundrum
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