competent cell preparation

Gorilka Gorilka at
Mon Oct 30 10:08:17 EST 1995


Dear Lee:

Although I am not exactly sure what publication do you keep in mind, I
would suggest to check PNAS 86 (1989) 2172. The method describes
PEG-mediated transformation. The cells are being stored in LB-PEG-DMSO.
This method proved to be virtually foolproof in my hands. For more recent
reference for this method you can look up Methods in Enzymology 218 P.621.

Regards,
M.A.



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