HELP-33P LABEL IN GELSHIFT EXPERIMENTS

Sasha Kraev kraev at bc.biol.ethz.ch
Mon Oct 30 13:33:57 EST 1995


Eric, 
the answer depends very much how thick your gels are. With completely 
dried 0.5 mm gels there is almost no difference between 32P and 33P,
where intensity is concerned. However, the latter becomes increasingly 
weak when anything separates the gel from the film. In a wet gel of the 
same thickness (0.5 mm) the difference is about 5-fold, and will be
greater with thicker gels. Using 33P with 1.5.mm gels is plain 
impractical. 

Hope this helps,

-------------- next part --------------
Alexander Kraev, PhD                     Internet:    kraev at bc.biol.ethz.ch
> Lab.of Biochemistry III                            Phone:      0041-1-632-31-47
> Swiss Federal Inst. Of Technology      FAX:           0041-1-632-12-13
> Universitaetsstr. 16                      URL: http://129.132.39.25/kraev.html/
> CH-8092 Zurich


More information about the Methods mailing list